Estimation of Effective Volume of HPLC Alkyl Bonded Phases by means of Macromolecular Probes
نویسندگان
چکیده
A method for estimation of volume of alkyl bonded phases for high performance liquid chromatography (HPLC) is presented. It is based on application of macromolecular probes, which are able to elute from the HPLC column without entering the bonded groups and thus to monitor their volume. The appropriate mathematical expression of results were looked for and it was concluded that the optimum represent polynomials of second degree. Key –Words: Polymer Liquid Chromatography, polynomial regression, multi-agent systems
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